HKPG Heat Killed P. gingivalis

HKPG Heat Killed P. gingivalis
KPG is a freeze-dried heat-killed preparation of the periodontopathic bacteria Porphyromonas gingivalis. In chinese hamster ovary (CHO) cells cotransfected with CD14 and TLR2, exposure to HKPG induces the activation of NF-κB dose dependently indicating that TLR2 plays an important role in the recognition of these bacteria. Expression of TLR4 failed to enhance the response to HKPG suggesting that either the whole bacterial components of P. gingivalis are not recognized by TLR4 or some components of these bacteria inhibit TLR4-mediated activation [1].

Specificity: TLR2 agonist

Working concentration: 10⁶-10⁸ cells/ml

Solubility: Insoluble (resuspended HKPG results in a cloudy suspension)
10¹⁰ freeze-dried cells of Heat Killed Porphyromonas gingivalis (HKPG)
• 1.5 ml sterile endotoxin-free water

HKPG is provided lyophilized and shipped at room temperature. Store at 4°C. Upon resuspension, prepare aliquots of HKPG and store at 4°C for short term storage or -20°C for long storage. Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.

Recent articles using InvivoGen HKPG

2012 - Immunity, 36(3):464-476
An NLRP7-containing inflammasome mediates recognition of microbial lipopeptides in human macrophages.
Khare S, Dorfleutner A, Bryan NB, Yun C, Radian AD, de Almeida L, Rojanasakul Y, Stehlik C
1.Yoshimura A. et al., 2002. Lipopolysaccharides from periodontopathic bacteria Porphyromonas gingivalis and Capnocytophaga ochracea are antagonists for human toll like receptor 4. Infect Immun.70(1):218-25.