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- invivogen
- tlrl-hkpg
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- 2025年08月31日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
研卉生物
- 细胞形态：
10e10 cells
- 生长状态：
106-108 cells/ml
- 规格：
10e10 cells
KPG is a freeze-dried heat-killed preparation of the periodontopathic bacteria Porphyromonas gingivalis. In chinese hamster ovary (CHO) cells cotransfected with CD14 and TLR2, exposure to HKPG induces the activation of NF-κB dose dependently indicating that TLR2 plays an important role in the recognition of these bacteria. Expression of TLR4 failed to enhance the response to HKPG suggesting that either the whole bacterial components of P. gingivalis are not recognized by TLR4 or some components of these bacteria inhibit TLR4-mediated activation [1].
Specificity: TLR2 agonist
Working concentration: 106-108 cells/ml
Solubility: Insoluble (resuspended HKPG results in a cloudy suspension)
1010 freeze-dried cells of Heat Killed Porphyromonas gingivalis (HKPG)
• 1.5 ml sterile endotoxin-free water
HKPG is provided lyophilized and shipped at room temperature. Store at 4°C. Upon resuspension, prepare aliquots of HKPG and store at 4°C for short term storage or -20°C for long storage. Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.
Recent articles using InvivoGen HKPG
2012 - Immunity, 36(3):464-476
An NLRP7-containing inflammasome mediates recognition of microbial lipopeptides in human macrophages.
Khare S, Dorfleutner A, Bryan NB, Yun C, Radian AD, de Almeida L, Rojanasakul Y, Stehlik C
1.Yoshimura A. et al., 2002. Lipopolysaccharides from periodontopathic bacteria Porphyromonas gingivalis and Capnocytophaga ochracea are antagonists for human toll like receptor 4. Infect Immun.70(1):218-25.
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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
their contents (P. St. Lawrence), and are conveniently stored for future use. 8. Heatshock. For heatshock, a 60°C water bath is preferable to a hot-air oven. (Greater latent heat, better heat transfer, more stable). Allow at least 30 minutes at 60°C to assure
preparations are incubated with serial dilutions of antiserum for 1 hour at 37o C. The agglutination of live cells is characterized by a coarse granular bacterial clumping, as opposed to the finer granular clumping of heat-killed P. aeruginosa with homologous
Hints and precautions for the care, feeding and breeding of Neurospora
by substituting 0.7 g K2HP04 and 0.5 g KH2HP04 per liter for the monobasic salt in the original formula (1963 N.N. 4:21-25). The formula in Davis and de Serres, p. 86, incorporates this change. The trace elements used for Vogel's medium N are suitable for SC
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