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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 库存:
大量
- 供应商:
联硕生物
- CAS号:
66575-29-9
- 规格:
1瓶
一般描述
生化/生理作用
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Store in a universal at room temperature 25m l De-ionised formamide (Sigma F7508) 15 m l probe eg. Appligene/Oncor #CP5040-B.5 (biotin labelled) or label your own probe (~10
DNA extraction from Mutation Detecti
sodium acetate (pH 5.2) and 5-10µl glycogen (10mg/ml) used as a carrier, followed by incubation at -70¡ãC for 30 min to precipitate DNA. After Centrifugation at 12000-¡Á g for 10minutes, the supernatant was removed and the DNA pellet was rinsed with 100µl
DNA extraction from Mutation Detecti
when eluting DNA from polyacrylamide gels. BioTechniques. 29 :694-696 5.Hawkins, J.R. 1997. Finding mutation, P.56-69. Oxford University Press, New York. 6.Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular cloning: A Laboratory Manual, 2nd ed. CSH
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