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文献和实验Generation of a Stable Cell Line for Constitutive miRNA Expression
Here, a protocol is described to generate stable cell lines for constitutive expression of single cellular or viral miRNA precursors in absence of infection. The procedure comprises cloning of the precursor sequence, generation of the lentiviral expression vector
Determination of HSV-1 Infectivity by Plaque Assay and a Luciferase Reporter Cell Line
of infectivity in a given volume. Due to advances in the understanding of viral gene expression, transactivator-promoter pairs have been identified which can be used in transgenic cell lines as reporters of viral infection. Even though such “cellular reporter
Robust Cell Line Development Using Meganucleases
Cell line development for protein production or for the screening of drug targets requires the reproducible and stable expression of transgenes. Such cell lines can be engineered with meganucleases, sequence-specific endonucleases
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