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- Sino Biological
- 14884-HNCB
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：DPYS蛋白, DPYS protein
蛋白构建：A DNA sequence encoding the human DPYS (NP_001376.1) (Met1-Pro519) was fused with two additional amino acids (Gly&Pro) at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly
蛋白分子量：The recombinant human DPYS consists of 521 amino acids and has a calculated molecular mass of 56.8 kDa. The recombinant protein migrates as an approximately 54 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001376.1
蛋白氨基酸序列：Met1-Pro519
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
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