Recombinant Human Pleiotrophin / PTN / HB-GAM Protein | 重组人 Pleiotrophin / PTN / HB-GAM 蛋白
- ￥1780 - 29180
- Sino Biological
- 北京
- 10288-HNAB
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Pleiotrophin / PTN / HB-GAM Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg/1 mg
| 规格： | 20 µg | 产品价格： | ￥1780.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥4480.0 |
| 规格： | 1 mg | 产品价格： | ￥29180.0 |
蛋白名称：Human Pleiotrophin / PTN / HB-GAM Protein
蛋白构建：A DNA sequence encoding the mature form of human PTN (NP_002816.1) (Met1-Asp168) was expressed.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human PTN (Cat:10288-HNAB) at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc (Cat:80344-R02H), The EC50 of rat SDC1-Fc (Cat:80344-R02H) is 0.35-0.81 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human PTN consists of 136 amino acids and predicts a molecular mass of 15.3 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002816.1
蛋白氨基酸序列：Met1-Asp168
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Comparative Protein Structure Modeling Using MODELLER
). (C ) Errors in regions without a template. The Cα trace of the 112–117 loop is shown for the X‐ray structure of human eosinophil neurotoxin (red), its model (green), and the template ribonuclease A structure (residues 111–117; blue). (D ) Errors
Intracellular Cytokine Staining Protocol
stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24 hours stimulation
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