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- Sino Biological
- 北京
- 13605-H20B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human PDE1C Protein (His & GST Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
蛋白名称：Human PDE1C Protein (His & GST Tag)
蛋白构建：A DNA sequence encoding the human PDE1C (Q8TAE4) (Met1-Glu634) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human PDE1C /GST chimera consists of 871 amino acids and has a calculated molecular mass of 100 kDa. The recombinant protein migrates as an approximately 97 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：Q8TAE4
蛋白氨基酸序列：Met1-Glu634
蛋白标签：N-GST & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
) in any given fraction have relevance to any human disease? If the protein has no obvious use as a drug target, it is ignored. If the protein is deemed relevant, one immediately has a lead molecule and a defined target. In cases where a single protein is eluted
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