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- Sino Biological
- 北京
- 12885-H20B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human MAX / MYC associated factor X Protein (His & GST Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
蛋白名称：MAX蛋白, MAX protein
蛋白构建：A DNA sequence encoding the human MAX (NP_002373) (Met1-Ser160) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human MAX consists of 397 amino acids and has a calculated molecular mass of 46.1 kDa. The recombinant protein migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002373
蛋白氨基酸序列：Met1-Ser160
蛋白标签：N-GST & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验作用。 2. Triton X-100在紫外波段下有光吸收（lambda max = 275 nm and 283 nm in methanol），因此，当缓冲液中存在Triton X-100时不能通过测定280nm光吸收来进行蛋白定量。 3. Triton X-100常与CHAPS等zwitterionic 去垢剂一起使用来纯化膜蛋白，使膜蛋白保持其天然构象。 4.Triton X-100可以提高真核细胞细胞膜的通透
is to put samples in multiple eppendorfs and spin in cold room at max for 2 min.Binding of Fusion Protein to Beads1)Pipette 400 μl of GST-Sepharose into Eppendorf2)Spin beads 15 sec @ 8,000 RPM3)Wash beads 2x with 4℃ NETN4)Resuspend pellet in 320 μl of NETN (Final
Purification of GST fusion proteins in E.coli GST融合蛋白纯化--纯化小量蛋白Sugden lab,McArdle Laboratory for Cancer Research,University of Wisconsin-Madison Medical SchoolSmall scale fusion protein preparationGrow 5ml culture o/n in TB with amp.Add o/n culture
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