Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
50 µg
重组人 AMPK (G1/B2/A2) 异源三聚体 蛋白(产品说明)
蛋白名称:Human AMPK (G1/B2/A2) Heterotrimer Protein
蛋白构建:A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met & Met & His
蛋白分子量:The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:P54619 & Q9Y478 & P54646
蛋白氨基酸序列:Met1-Pro331 & Met1-Ile270 & Met1-Arg552
蛋白标签:N-GST & N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Wang, T; et al. The AMPK-HOXB9-KRAS axis regulates lung adenocarcinoma growth in response to cellular energy alterations. Cell reports, Pubmed: 36001969