- ￥4650 - 5820
- Sino Biological
- 北京
- CT006-H0907B
- 2026年01月26日
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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥4650.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥5820.0 |
重组人 AMPK (G1/B2/A2) 异源三聚体 蛋白（产品说明）
蛋白名称：Human AMPK (G1/B2/A2) Heterotrimer Protein
蛋白构建：A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met & Met & His
蛋白分子量：The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号：P54619 & Q9Y478 & P54646
蛋白氨基酸序列：Met1-Pro331 & Met1-Ile270 & Met1-Arg552
蛋白标签：N-GST & N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human AMPK (G1/B2/A2) Heterotrimer Protein
蛋白构建：A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met & Met & His
蛋白分子量：The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号：P54619 & Q9Y478 & P54646
蛋白氨基酸序列：Met1-Pro331 & Met1-Ile270 & Met1-Arg552
蛋白标签：N-GST & N-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Wang, T; et al. The AMPK-HOXB9-KRAS axis regulates lung adenocarcinoma growth in response to cellular energy alterations. Cell reports, PMID: 36001969
2, Xie, F; et al. Targeting FOXM1 condensates reduces breast tumour growth and metastasis. Nature, PMID: 39814884
2, Xie, F; et al. Targeting FOXM1 condensates reduces breast tumour growth and metastasis. Nature, PMID: 39814884
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重组人 AMPK (G1/B2/A2) Heterotrimer蛋白
￥4650 - 5820
