- ￥3870
- Sino Biological
- 北京
- 10909-H08B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human ENTPD3 / NTPDase3 / CD39L3 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
重组人 ENTPD3 / NTPDase3 / CD39L3 蛋白 (His标签)（产品说明）
蛋白名称：Human ENTPD3 / NTPDase3 / CD39L3 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >70,000 pmol/min/µg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 44
蛋白分子量：The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：O75355
蛋白氨基酸序列：Gln44-Pro485
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Human ENTPD3 / NTPDase3 / CD39L3 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >70,000 pmol/min/µg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 44
蛋白分子量：The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：O75355
蛋白氨基酸序列：Gln44-Pro485
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验相关实验
)； (7) 重复(1)-(5)步，pH梯度为X-0.6；X-0.3；X；X+0.3；X+0.6；X+1。 (8) 观察胶体金颜色变化直到室温下放置2小时，记录仍保持红色的最低pH。 注意： 1．如胶体金粒子直径较小（3～6nm），加NaCl需放置较长时间（几个甚至十几个小时）后才能观察到颜色变化。必要时可放大反应体积（1 ml胶体金），并借助离心来判断。 2．有些蛋白最佳pH范围较窄，设置的梯度不能太大。 5、最小蛋白浓度的确定 (1) 用0.22 mm微孔滤膜过滤或高速
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重组人 ENTPD3 / NTPDase3 / CD39L3蛋白 (His 标签)
￥3870
