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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human/Mouse/Rat/Cynomolgus/Rhesus Activin A/INHBA Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥22680.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥1790.0 |
| 规格: | 20.00 µg | 产品价格: | ¥1980.0 |
| 规格: | 50.00 µg | 产品价格: | ¥3480.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4980.0 |
蛋白名称:Human / Mouse / Rat / Cynomolgus / Rhesus Activin A / INHBA Protein
蛋白构建:A DNA sequence encoding mature form of human / mouse / rat / cynomolgus / rhesus INHBA (NP_002183.1) (Gly311-Ser426) was expressed. The mature form sequences of the human, mouse, rat, cynomolgus and rhesus INHBA are identical.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 4-16ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gly 311
蛋白分子量:The recombinant human / mouse / rat / cynomolgus / rhesus INHBA consists of 116 amino acids and predicts a molecular mass of 13 kDa.
蛋白NP号:NP_002183.1
蛋白氨基酸序列:Gly311-Ser426
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Lai, YJ; et al. Naringin activates semaphorin 3A to ameliorate TGF-β-induced endothelial-to-mesenchymal transition related to atrial fibrillation. Journal of cellular physiology, PMID: 38501506
3, Chai, C; et al. Explosive cytotoxicity of 'ruptoblasts' bridges hormonal surveillance and immune defense. bioRxiv : the preprint server for biology, PMID: 40236000
4, Dong, S; et al. RNA m6A dynamics promote transcription and RNA stability of bivalent genes during iPSC-induced generation of human lung progenitors. Cell reports, PMID: 40483690
下调不能说明活性降低,因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外,还要做磷酸化的GSK3-beta的蛋白表达。 (2)应该活性形式和非活性形式都做。一般来讲,GSK3-beta在Ser9位点磷酸化之后活性收到抑制,而在216位点磷酸化之后,其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了,另外还要同时检测GSK3-beta的总蛋白表达,这样才能全面的说明问题。
扫描仪中,都采用机械式的二维X,Y线性扫描技术实现,即X,Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置,由于驱动系统的频率限制,驱动器的扫描惯性大,使得扫描效率低,分析时间相当长;并且往复行程长,对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描,我们的实验装置设计如图2,采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描,Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中,对于f-è物镜,满足x=2fè(è为振镜的摆动角度,f为物镜焦距)的线性
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
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