- ￥5170
- Sino Biological
- 81349-R08H
- 2025年08月12日
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- 文献和实验
- 技术资料
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100 µg
蛋白名称：NKp30/NCR3蛋白, NKp30/NCR3 protein
蛋白构建：A DNA sequence encoding the rat Ncr3 (CAD23066.1) (Met1-Ser147) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat NCR3-His(Cat:81349-R08H) at 10μg/mL (100μL/well) can bind rat B7-H6-Fc (Cat:81348-R02H), the EC50 of rat B7-H6 -Fc is 1-20ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ile 19
蛋白分子量：The recombinant rat Ncr3 consists 140 amino acids and predicts a molecular mass of 15.2 kDa.
蛋白NP号：CAD23066.1
蛋白氨基酸序列：Met1-Ser147
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验. In addition, certain subclasses of rat IgG have been found to interact efficiently with human Fc receptors and the human complement system. More important perhaps in practical terms is that, when grown as an ascites, up to 30 mL of fluid containing 1–5 mg/mL
ChIP是一种强大的确定蛋白或者组蛋白修饰在基因组上定位的实验方法。染色质被分离出来后采用抗体与抗原的结合来判定目的蛋白是否结合在特定的DNA序列上或者判定目的蛋白结合位点在全基因组范围的分布（微阵列或DNA序列）。这种方法具有空间性与时效性。该实验设计为如何在细胞中进行ChIP实验提供了详细的步骤。1. 交联和细胞收获甲醛可以将蛋白质交联到DNA上。交联结果的好坏决定于交联时间的把握。我们建议样品交联的时间一般为2-30分钟。过度的交联会减少抗原的结合性和超声断裂的效率。抗原决定簇也会被掩盖
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
Step 3: 72.0ºC for 00:30 Cycle 3: ( 80X) Step 1: 55.0ºC for 00:10 Increase setpoint temperature after cycle 2 by 0.5ºC Melt curve data collection and analysis enabled
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