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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Syndecan-4 / SDC4 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Syndecan-4蛋白, Syndecan-4 protein
蛋白构建:A DNA sequence encoding the mouse SDC4 (O35988)(Met1-Val146) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human MDK (Cat:10247-HNAB) at 10 μg/ml (100 μl/well) can bind mouse SDC4-Fc (Cat:50726-M02H) with a linear range of 0.16-1.25 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 24
蛋白分子量:The recombinant mouse SDC4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 364 amino acids and has a predicted molecular mass of 40.5 kDa. The apparent molecular mass of the protein is approximately 33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:O35988
蛋白氨基酸序列:Met1-Val146
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白,兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G,也可根据结合力表格进行选择,详见资源信息部分 3、基质—琼脂糖还是磁珠? 琼脂糖 磁珠 优点 简单易用,无需辅助设备 操作简单 孔径大,直径大(50-150
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐affinity tag antibodies for development and determination of protein display, 2624B (Cell Signaling Technology) Hybridization chamber (Corning, product no. 2551
一抗的物种来源。 抗体是根据一抗的物种来源的字母表顺序排列。比如,如果一抗是小鼠来源的,就找“ anti-mouse ”。 说明:目录中提供了抗叙利亚仓鼠和抗亚美尼亚仓鼠的抗体。为了正确的选择二抗,必须知道一抗是在哪种仓鼠中制备的。 第三步: 在标有“ Host ”这一栏中选择二抗的物种来源。 二抗的物种来源在抗体描述的开始部分,选择二抗的物种来源应考虑几点: 1) 个人的取向或经验。 二抗的质量问题与物种特异性没有关系。2 )与蛋白A 和蛋白G 结合。兔抗体能很好地与蛋白
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