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50 µg/500 µg
| 规格: | 50 µg | 产品价格: | ¥2310 |
|---|---|---|---|
| 规格: | 500 µg | 产品价格: | ¥16740 |
蛋白名称:CD3D & CD3E Heterodimer蛋白, CD3D & CD3E Heterodimer protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 22 & Asp 22
蛋白分子量:The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
蛋白NP号:P04235 & P22646
蛋白氨基酸序列:0
蛋白标签:C-Flag & C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验CD26 分子 CD26 常用单克隆抗体或代号: 5.9,Tal 主要表达细胞: Ta,Ba,Mac [NL] 分子质量(kDa)和结构: gp110 功 能: 二肽酰酶Ⅳ(DPPⅣ),HIV另一类受体,参与T细胞活化,腺苷脱氨酶结合蛋白 CD26 Aka dipeptidylpeptidase IV, adenosine deaminase complexing protein
PFHM-II (Protein-free Hybridoma Medium)是不包含有蛋白质的单克隆抗体生产培养基。如果作为杂交瘤生产培养基使用,PFHM-II可能需要补充低水平的血清。HYBRIDOMA-SFM即是杂交瘤生产培养基,也是单克隆抗体生产培养基。它包含低水平的蛋白质。 22.在High Five无血清培养基中去污剂的浓度是多少? High Five无血清培养基中去污剂的浓度:0.025 g/L Tween-80,1.0 g/L Pluronic Poly
9.26 Nat Commun:发现细菌中的 Crispr-Cas9 防御系统
图位克隆技术获得了调控水稻顶部小穗退化的新基因 DPS1 , 该基因编码的蛋白可参与活性氧的清除。通过实验发现, dps1 突变体与脂肪酸代谢和活性氧稳态相关的生物过程受到显著影响,并且蜡和角质生物合成相关的关键基因下调表达。该研究有助于了解水稻生殖发育的机理,对高产水稻品种的选育具有重要意义。原文检索: DEGENERATED PANICLE AND PARTIAL STERILITY 1 (DPS1) encodes a CBS domain containing protein
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