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- Sino Biological
- 90226-C08H
- 2025年08月12日
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- 技术资料
- 规格：
100 µg
蛋白名称：SIRP gamma/SIRPG蛋白, SIRP gamma/SIRPG protein
蛋白构建：A DNA sequence encoding the cynomolgus SIRPG (Met1-His360) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 29
蛋白分子量：The recombinant heterodimer of cynomolgus SIRPG comprises 343amino acids and has a calculated molecular mass of 38.1 KDa. The apparent molecular mass of the protein is approximately 45 KDa respectively in SDS-PAGE.
蛋白NP号：0
蛋白氨基酸序列：Met1-His360
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
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