重组大鼠 IFNGR / IFNGR1 蛋白 (Fc标签)(产品说明)
蛋白名称:Rat IFNGR / IFNGR1 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the rat IFNGR (Q6P6T3) (Met1-Ser241) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized Rat IFNGR1 hFc (Cat:80182-R02H) at 2 μg/ml (100 μl/well) can bind Rat Interferon Gamma hFc(Cat:80234-R02H), Biotinylated the EC50 of Rat Interferon Gamma hFc, Biotinylated is 60-300 ng/mL.2. Measured by its ability to inhibit rat IFNγ-mediated protection of L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.5-4 ng/mL in the presence of 10 ng/mL of recombinant rat IFN-gamma.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 17
蛋白分子量:The recombinant rat IFNGR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 466 amino acids and has a predicted molecular mass of 52.4 kDa. The apparent molecular mass of the protein is approximately 117 and 65-80 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q6P6T3
蛋白氨基酸序列:Met1-Ser241
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.