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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse SHH / Sonic hedgehog Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
5.00 µg/20.00 µg/100.00 µg
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥1080.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3480.0 |
蛋白名称:Sonic Hedgehog蛋白, Sonic Hedgehog protein
蛋白构建:A DNA sequence encoding the mouse SHH (Q62226) (Met1-Gly198) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 5-40 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 25
蛋白分子量:The recombinant mouse SHH comprises 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:Q62226
蛋白氨基酸序列:Met1-Gly198
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验人Sonic Hedgehog(SHH)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Sonic Hedgehog ( SHH )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 SHH 单抗包被于酶标板上,标准品和样品中的 SHH与单抗结合,加入生物
Application of Sonic Hedgehog to the Developing Chick Limb
Here, we describe methods for applying Sonic hedgehog (Shh) to developing chick limbs. The Sonic hedgehog gene is expressed in the polarizing region, a signaling region at the posterior margin of the limb bud and application of Shh-expressing
SYendocrine 想请问各位大虾,sonic hedgehog通路的特异性激活剂是什么? (附加:另外有没有哪位知道该通路在肾小球系膜细胞中是否表达,如果想测是否表达通过RT-PCR测SHH,通过Elisa法测shh蛋白就行了么?) 请各位大虾不吝赐教!! 大鹏鸟 好像只有SHH能激活,没看到有文献说有其他激活剂的。 想测表达与否还是通过传统的方法,RT-PCR ,WESTERN,ELISA
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