- ￥1080 - 19380
- Sino Biological
- 北京
- 50385-MNAC
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse β-NGF / Beta-NGF Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg/1 mg
| 规格： | 20 µg | 产品价格： | ￥1080.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥2980.0 |
| 规格： | 1 mg | 产品价格： | ￥19380.0 |
蛋白名称：NGF蛋白, NGF protein
蛋白构建：A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.
表达宿主：CHO Stable Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant mouse NGF consists of 121 amino acids and has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001106168.1
蛋白氨基酸序列：Ser122-Gly241
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验2, Lu, F; et al. Pharmacological Ischemic Conditioning with Roxadustat Does Not Affect Pain-Like Behaviors But Mitigates Sudomotor Impairment in a Murine Model of Deep Hind Paw Incision. papers.ssrn.com
3, Liang, ZJ; et al. NGF monoclonal antibody DS002 alleviates chemotherapy-induced peripheral neuropathy in rats. Acta pharmacologica Sinica, Pubmed: 35468993
4, Xiong Gao, A; et al. Luteolin stimulates the NGF-induced neurite outgrowth in cultured PC12 cells through binding with NGF and potentiating its receptor signaling. Food & function, Pubmed: 34704574
5, Lian, WY; et al. The Role of Klotho Protein Against Sevoflurane-Induced Neuronal Injury. Neurochemical research, Pubmed: 34498160
6, Yuan, Q; et al. Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system. Biosci. Biotechnol. Biochem., Pubmed: 28394221
7, Arioz, I; et al. Biocompatible Electroactive Tetra(aniline)-Conjugated Peptide Nanofibers for Neural Differentiation. ACS Appl Mater Interfaces, Pubmed: 29232108
8, Hu, Z; et al. DLK mediates the neuronal intrinsic immune response and regulates glial reaction and neuropathic pain. Exp. Neurol., Pubmed: 31494101
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
attenuator 有一个问题一直没有查出相关资料或信息。 就是：β globin 蛋白在血液组织之外的细胞有没有表达，能不能RT检测出transcripts或western检测出protein。 为了这个去重复实验，条件不允许，但前人肯定有做过的，教科书里没有介绍，只介绍了时间特异性（胚胎到成体），没有介绍组织特异性——除了造血组织（或成红祖细胞）之外，在其他组织里，β globin是不是就沉默了，它那一簇基因是不是从它LCR
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