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- 规格:
100 µL
抗体种属:Rat
抗体靶点:FCGR3A
抗体应用:ELISA
抗原货号:80017-R08H
抗原描述:Recombinant Rat CD16a / FCGR3A protein (Catalog#80017-R08H)
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体标记:
抗体制备:This antibody was obtained from a rabbit immunized with purified, recombinant Rat CD16a / FCGR3A (Catalog#80017-R08H; ABO70343.1; Met1-Pro200).
抗体特异性:Rat CD16a / FCGR3A
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
HIV-1-Specific Antibody-Dependent Cellular Cytotoxicity (ADCC)
-1-infected cells thereby decreasing viral load (1 –5 ) . The effector cells for ADCC are natural killer (NK) cells, that mediate lysis in a non-MHC restricted fashion. NK cells expressing CD16+ low affinity Fc-receptors bind to antibody which specifically
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