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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Mouse CD16 / FCGR3 protein (Catalog#50326-M08H)
- 亚型:
Rabbit IgG
- 形态:
液体
- 保存条件:
-20℃ 至 -80℃
- 克隆性:
单克隆
- 标记物:
PE
- 适应物种:
Mouse
- 保质期:
12个月
- 抗原来源:
50326-M08H
- 目录编号:
50326-R002-P
- 级别:
免疫学试剂
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Rabbit
- 应用范围:
FCM
- 浓度:
详见说明书
- 靶点:
FCGR3
- 抗体英文名:
FCGR3 Antibody (PE), Rabbit MAb
- 抗体名:
FCGR3 Antibody (PE), Rabbit MAb
- 规格:
25 Tests/100 Tests
| 规格: | 25 Tests | 产品价格: | ¥300 |
|---|---|---|---|
| 规格: | 100 Tests | 产品价格: | ¥700 |
抗体种属:Mouse
抗体靶点:FCGR3
抗体应用:FCM
抗原货号:50326-M08H
抗原描述:Recombinant Mouse CD16 / FCGR3 protein (Catalog#50326-M08H)
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体标记:PE
抗体制备:This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; Catalog#50326-M08H; P08508-1; Met1-Thr215) and conjugated with PE under optimum conditions, the unreacted PE was removed.
抗体特异性:Mouse CD16 / FCGR3
抗体保存:This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
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文献和实验到的细胞类型表达FcR,同时反应抗体含有Fc段,都应该考虑进行Fc 阻断,来确保实验的特异性,减少非特异的产生。 那么,具体哪些细胞类型需要做Fc阻断? 首先,按FcR所结合的Ig种类不同可将其分为五类,即IgG(FcγR)、IgE(FcεR)、IgA(FcαR)、IgM(FcμR)和IgD(FcδR)。 IgG类型抗体的Fc受体是FcγR,依据和Fc片段亲和力的强弱,FcγR又可以被分为三个亚家族:FcγRI(CD64)、FcγRII(CD32)和FcγRIII(CD16)以及进一步还可以分为不同
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
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