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- Sino Biological
- 10148-MM14
- 2025年08月12日
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- 规格：
100 µL
抗体种属：Human
抗体靶点：PECAM1
抗体应用：FCM
抗原货号：10148-H08H
抗原描述：Recombinant Human CD31 / PECAM-1 protein (Catalog#10148-H08H)
抗体宿主：Mouse
抗体Ig类型：Mouse IgG1
抗体纯化方法：Protein A
抗体制备：This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM-1 (rh CD31 / PECAM-1; Catalog#10148-H08H; EAW94208.1; Met1-Lys601). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性：Human CD31 / PECAM-1
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验CD31 分子 CD31 常用单克隆抗体或代号： SG134,TM3,HEC- 75;(PECAM、EndoCAM) 主要表达细胞： Pt,M,G,B,NK,Tsub [AS] 分子质量(kDa)和结构： gp140,血小板GPⅡa(IgSF) 功 能： 嗜同性或嗜异性(与CD38互为受体)粘附,炎症,En功能 CD31 Aka
做不出来，后来也没有尝试。步骤：(1)水化(2)抗原修复：枸橼酸(PH = 6.0)溶液中，微波炉中火5 min后，室温冷却30 min，0.01M PBS漂洗5 min×3次。(3)破膜：50 μl的0.2% Triton X - 100，室温静置30 min，0.01M PBS漂洗5 min×3次。(4)封闭(5)孵一抗：滴加一抗(血管密度检测为: CD31 1:200)，每张脑片50 μl，置于4℃冰箱过夜(15 h)。(6)复温(7)孵二抗：50 μl的山羊抗小鼠488(1:1000)，室温
Surrogate Markers of Angiogenesis and Metastasis
of intratumoral microvessel density (IMD) of the primary tumor by using specific markers for endothelial cells such as factor VIII-related antigen (FVIII-RA), anti-CD31 (platelet endothelial cell adhesion molecule, PECAM), or anti-CD34 antibodies (19 ,22
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