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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Rat aldehyde dehydrogenase,ALDH ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.764 2.753 2.759 2.560
10 2.159 2.042 2.101 1.902
5 1.529 1.512 1.521 1.322
2.5 1.045 1.018 1.032 0.833
1.25 0.682 0.656 0.669 0.470
0.625 0.423 0.410 0.417 0.218
0.312 0.312 0.303 0.308 0.109
0 0.201 0.197 0.199
Rat aldehyde dehydrogenase,ALDH ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Rat aldehyde dehydrogenase,ALDH ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of rat ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 100
Range % 91-105
1:2 Average % 98
Range % 94-103
1:4 Average % 96
Range % 90-100
1:8 Average % 98
Range % 95-102
Rat aldehyde dehydrogenase,ALDH ELISA Kit
回收率:The recovery of rat ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 86-95
EDTA plasma (n=4) 91 87-96
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
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