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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人6-羟多巴胺(6-OHDA)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human 6-hydroxydopamine,6-OHDA ELISA Kit
产品货号:YM-KJ1345
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人6-羟多巴胺(6-OHDA)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人6-羟多巴胺(6-OHDA)ELISA试剂盒 试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验方法:①通过在大鼠的黑质或内侧前脑束注射6羟多巴胺(6-OHDA)②通过对模型动物(如:猴、小鼠、猪等)进行1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTR)的管饲法和立体定位注射等方法。前者是较精典的模型制备的方法,也是在国内较早开展的,但是其存在:1)神经元的毁损出现的较早,这与临床PD病人的中脑黑质多巴胺能神经元的渐进性死亡不同2)近距离的毁损很难把握毁损的程度,这使得采用这种方法获取部分损毁的模型的成功率很低;较之前者采用MPTP管饲法和立体定位注射方法,在国内最近几年才运用到帕金森动物
enolase 非神经元性烯醇化酶 NP neuropeptide 神经肽 NPY neuropeptide Y 神经肽Y NSE neuron-specific enolase 神经元特异性烯醇化酶 NT neurotensin 神经降压肽 OD optical density 光密度 6-OHDA 6-hydroxydopamine 6-羟多巴胺 ORF open reading frame 开放读框 ORT optimum
ALT,测定肝细胞的MDA含量;同步测定96孔板中培养肝细胞的MTT反应。根据检测结果制备H2O2诱导肝细胞损伤的量效和时效曲线,选择最适损伤浓度和损伤时间制备肝细胞的损伤模型,同时设溶媒对照组,每组至少设3个复孔。4、检测指标(1)AST、ALT的测定 培养的肝细胞经离心(1 800 r·min-1,10 min)后收集上清,按试剂盒说明书步骤测定上清液中AST和(或)ALT活性,结果以U·(106 cell)-1表示。(2)肝细胞增殖试验 采用MTT比色法。(3)肝细胞谷胱甘肽过氧
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