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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人胰激肽原酶(PK)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human pancreatickininogenase,PK ELISA Kit
产品货号:YM-KJ0971
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人胰激肽原酶(PK)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人胰激肽原酶(PK)ELISA试剂盒 试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验体可通过传递信息影响目标细胞的功能,激活细胞信号通路,在免疫、凝血、肿瘤等生理病理过程中发挥作用。外泌体可以作为临床诊断中的生物标志物或疾病治疗的靶点,同时也可以作为药物载体,参与神经疾病和癌症等多种疾病的病理过程。 三、关键步骤 — 外泌体分离 外泌体的分离纯度对外泌体的功能研究非常重要,但是外泌体的分离一直是困扰外泌体研究人员的一个难题。虽然分离外泌体方法有很多种,包括差速超速离心法(超离法)、超滤法、密度梯度离心法、免疫磁珠法、试剂盒法等。 但目前
相关专题 细胞因子是免疫 反应中分泌的一类蛋白质,机体的免疫反应通常是由多个细胞因子控制的。许多生物公司都在开发各种用于同时检测几十种细胞因子的试剂盒。文中罗列了几种市面上常见的免疫磁珠检测的产品及其性能比较。 细胞因子 是机体免疫反应中不同细胞分泌的一类小蛋白质。例如,当白细胞介素4(IL-4)单独发挥作用时,它使得B细胞增殖,并生产出特定类型的抗体。但干扰素-γ(IFN-γ)同时存在时,会减少B细胞的数目并改变它们产生
In Vivo Testing of Drug-Linker Stability
and metabolites entities. This chapter focuses on the key analytical methods (ELISA immunoassays, TFC-MS/MS, and HRMS) used to support the PK profiles assessment of the three entities, allowing the characterization of ADC “drug-linker stability”.
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