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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人全段甲状旁腺素(i-PTH)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human intact Parathormone ,i-PTH ELISA Kit
产品货号:YM-KJ0837
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人全段甲状旁腺素(i-PTH)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人全段甲状旁腺素(i-PTH)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验北京时间 2022 年 2 月 9 日,中国科学院深圳先进技术研究院脑认知与脑疾病研究所、深港脑科学创新研究院杨帆课题组在 Nature Communications 上发表题为 An optogenetic approach for regulating human parathyroid hormone secretion 的最新研究成果。 研究团队历时 5 年,创新性地将光遗传技术运用于甲状旁腺素(Parathyroid Hormone, PTH)的分泌调控,通过精准节律性调节
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人甲状旁腺激素 (I-PTH)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 I-PTH 单抗包被于酶标板上,标准品和样品中的 I-PTH与单抗结合,加入生物素化的抗人 I-PTH
甲状旁腺素(PTH)是甲状旁腺主细胞合成和分泌的多肽激素。它通过骨和肾来实现升高血钙,降低血磷的作用:①对骨的作用。PTH能动员骨钙入血,使血钙升高。它可作用于骨细胞膜系统,促使Ca 2 转运入细胞外液,这是PTH动员骨钙入血的快速效应,仅数分钟内即可发生。此外,还可加强破骨细胞的活动,使骨钙大量入血,这一效应为延缓效应,在PTH作用后12~14小时开始,几天或几周后达高峰。两个效应互相配合,不仅能迅速升高血钙,而且能维持很长时间。②对肾的作用。PTH能抑制近球小管对磷酸
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