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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
远慕生物
- 检测范围：
科研实验
- 检测方法：
双抗体夹心酶联免疫法(ELISA)
- 样本：
血液、尿，粪便，脑脊液，胸腹水，前列腺液，精液，阴道分泌物等
- 规格：
96T/48T
人抗精子抗体(AsAb)ELISA试剂盒说明书
上海远慕生物做实验试剂，专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂！欢迎来电咨询订购：Elisa试剂盒现货低价！特价促销！
Human anti-sperm antibody,AsAb ELISA Kit
产品货号：YM-KJ0805
规格：96T/48T
欢迎来电咨询订购：Elisa试剂盒，人抗精子抗体(AsAb)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人抗精子抗体(AsAb)ELISA试剂盒试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围：
0.2IU/L - 6IU/L
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文献和实验d/C3的比值。（5）ELISA法检测AsAb试剂盒购自南京军区南京总医院。按说明书介绍的方法操作。2、结果（1）42例不育男性，经检测AsAb阴性者26例，AsAb阳性者16例，阳性率38.1%。其中血清抗精子抗体阳性者9例，精浆抗精子抗体阳性者7例(有两例血清抗精子抗体同时为阳性)。8例生育男性血清和精浆AsAb均为阴性。（2）不育男性及生育男性精浆C3d、C3及C3d/C3测定结果见表1。表1 不育男性及生育男性精浆C3d、C3及C3d/C3测定结果 生育组(n=8)不育组AsAb(－)n=26
www.westang.com 上海西唐生物科技公司 021-55229872 分装 ELISA 试剂盒 货号 品名 方法
。 ⑤精子泳动速度试验：在显微镜下测定精子在精液中运动的平均泳动速度。 ⑵精浆的临床化学与免疫学检查 ①酸性磷酸酶（acid phosphatase, ACP）：常用速率法测定。 ②乳酸脱氢酶-X同功酶（LD-X）：同工酶电泳分析法。 ③果糖测定：间苯二酚法。 ④抗精子抗体（Antispermatozoon antibodies, AsAb）的检测：精子凝集或制动试验，间接免疫荧光试验。 ⑶精液的微生物学检查：直接涂片革兰染色或抗酸染色，免疫
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