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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
小鼠淋巴细胞功能相关抗原3(LFA-3/CD58)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Mouse lymphocyte function associated antigen 3,LFA-3 ELISA Kit
产品货号:YM-KJ3243
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,小鼠淋巴细胞功能相关抗原3(LFA-3/CD58)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
小鼠淋巴细胞功能相关抗原3(LFA-3/CD58)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验人淋巴细胞功能相关抗原(LFA-1)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人淋巴细胞功能相关抗原 ( LFA-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人LFA-1 单抗包被于酶标板上,标准品和样品中的 LFA-1与单抗结合,加入生物素化的抗人
白细胞分化抗原及其功能 CD2 又称淋巴细胞功能相关抗原2(LFA 2)。CD2家族还包括CD48、CD58、2B4、L?9以及CDl50等分子。CD2分子胞膜外N端有1个IgSFV样区,近膜区有1个C2样区,胞质区富含碱性氨基酸和脯氨酸。CD2分子表达于胸腺细胞、T细胞和NK细胞。 与T细胞识别、黏附和活化有关CD分子的结构 人CD2分子以其V样结构域和配体CD58(LFA-3)结合:CD2与配体的相互识别
白细胞分化抗原及其功能 CD58 又称淋巴细胞功能相关抗原3(LFA-3),既是CD2的配体又属CD2家族成员,其胞膜外区有IgSF的V 样区和C2样区。 与T细胞识别、黏附和活化有关CD分子的结构 CD58分子有跨膜和GPI连接两种形式。其分布较为广泛,包括T细胞、B细胞、单核细胞、IX;、中性粒细胞、血小板和红细胞。在非造血细胞中如上皮细胞、内皮细胞、成纤维细胞也表达CD58。表达CD58的APC
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