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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human Alpha-fetoprotein,AFP ELISA Kit
PRINCIPLE OF THE ASSAYThis assay employs the quantitative sandwich enzyme immunoassay technique.
Antibody specific for AFP has been pre-coated onto a microplate. Standards and
samples are pipetted into the wells and any AFP present is bound by the
immobilized antibody. After removing any unbound substances, a Horseradish
Peroxidase (HRP)-conjugated antibody specific for AFP is added to the wells.
Following a wash to remove any unbound reagent, a substrate solution is added
to the wells and color develops in proportion to the amount of AFP bound in the
initial step. The color development is stopped and the intensity of the color is
measured.
Human Alpha-fetoprotein,AFP ELISA Kit
DETECTION RANGE1.563 ng/ml-100 ng/ml.
Human Alpha-fetoprotein,AFP ELISA Kit
SENSITIVITYThe minimum detectable dose of human AFP is typically less than 1.332 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest protein concentration that could be differentiated from zero. It was
determined the mean O.D value of 20 replicates of the zero standard added by
their three standard deviations.
Human Alpha-fetoprotein,AFP ELISA Kit
SPECIFICITYThis assay has high sensitivity and excellent specificity for detection of human
AFP. No significant cross-reactivity or interference between human AFP and
analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human AFP and all the analogues,
therefore, cross reaction may still exist.
3
PRECISION
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
LIMITATIONS OF THE PROCEDURE
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the
samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation
in binding.
This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all
factors have been tested in the Immunoassay, the possibility of
interference cannot be excluded.
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
癌、卵巢肿瘤、恶性畸胎瘤、胰腺癌、肠癌、肺癌等患者。 产甲胎蛋白的胃癌 (alpha-fetoprotein-producing gastric carcinoma, AFPGC) 是一种相对少见的胃癌 (GC)，最早由 Bourreille 等人 [6] 于 1970 年提出，高 AFP 水平的胃癌被称为 AFPGC [7]。AFPGC 约占 GC 的 2.7~8%[8]，也有报道占 GC 的 5.1%~15%[9]。AFPGC 是指经过病理或组织学检查确诊，伴有血清 AFP 升高，并可以排除肝炎
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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