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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
一年
- 英文名:
ORF cDNA clone sets
- 供应商:
GeneCopoeia
- 库存:
大量
GeneCopoeia是NCBI推荐的基因克隆供应商之一,提供多种基因克隆产品,以满足广大顾客多元化的需求。
基因和蛋白的过表达实验广泛用于基因组学、蛋白质组学、和系统生物学的研究。但是要获得即用型表达克隆需要复杂的克隆、验证和序列分析的过程。 GeneCopoeia提供多种表达系统、启动子克隆、融合标签及筛选标记,客户可以定制或购买预制的即用型表达克隆。所有ORF与cDNA克隆序列均经过验证,可用于即时表达。
GeneCopoeia提供人和小鼠即时表达的全长ORF克隆,OmicsLink™即用型 ORF表达克隆可以在体内体外即时表达,帮助客户更迅速地得到研究结果,更快地发表文章。
满足生物化学和细胞生物学科研的诸多需求
- 可以在大肠杆菌、哺乳动物细胞和其他常见细胞中表达
- 可以在无细胞体系中正确表达和翻译
- 提供蛋白纯化和生产需要的
- 有不同功能的蛋白标签供选择
- 可制作原位杂交探针,用以检测组织或器官中基因的表达
- 可用于shRNA和miRNA抑制基因的功能拯救实验
高通量筛选
高质量的表达克隆组合是大规模筛选的最佳选择,已被广泛地应用到功能基因组学,蛋白组学和系统生物学的前沿领域。
严格的质量控制体系
OmicsLink™ 即用型ORF表达克隆的源于经过严格筛选的人和小鼠全长基因,通过多个渠道比对、验证基因序列及注释信息,进行人工纠正,减少冗余、错误的序列。
OmicsLink™ 即用型ORF表达克隆是由全长cDNA克隆和高质量的人体组织cDNA文库作PCR模板构建的。
这些经过严格质量验证OmicsLink™ 即用型ORF表达克隆在投递前都经过严格的质量检测:
- 所有的ORF经过全长测序
- PCR扩增验证长度
- 质粒通过了酶切验证
简单多样的搜索选择

大量的蛋白融合标签选择
| 荧光标签 | eGFP, eYFP, eCFP, mCherry |
| 多功能标签 | HaloTag®, AviTag™ |
| 促溶解度和纯化标签 | His, SUMO, GST, MBP, Flag, 3xFlag |
| 抗体免疫共沉淀标签 | HA, cMyc |
Anti-tag antibodies available for easy detection
应用广泛的蛋白标签和科研前沿技术 |
- 慢病毒技术——能高效地把基因运送到几乎任何哺乳动物细胞,并高效表达蛋白。
- 内部核糖体进入位点(IRES)技术 — 可以使得在同一启动子驱动下被IRES 连接的两个基因高效共表达,靶基因和报告基因能够一起表达,且避免了蛋白融合标签对基因结构域的影响,可以有效地在宿主中展示靶基因的生物学活性。
- HaloTag® 技术 — 优秀的多功能标签,可共价并特异地接合多种配体,可在体外蛋白亲和纯化实验和细胞活体蛋白标记实验中发挥广泛的功能。
- AviTag™ 技术 — 基于特定位点生物素化一段标签,可以让融合蛋白可逆地结合生物素或链霉亲和素,广泛地应用于体外研究的蛋白固定化、纯化实验和细胞内的蛋白示踪实验。
- 无细胞技术 —能够成功地表达靶蛋白并容获得蛋白的天然折叠形式,可以表达对宿主细胞有毒性的蛋白。
多种载体可选的ORF克隆套装
GeneCopoeia除了提供单个克隆,还提供预制和定制的克隆套装,价格优惠,应用范围广。了解详情请点击联系我们或拨打4006-020-200。
全人类基因组克隆
如果您有什么问题,请致电4006-020-200
| 货号 | 物种 | 套装类型 | 基因数 | 载体 | 载体类型 | 价格 |
|---|---|---|---|---|---|---|
| OC-HCC-M02 | Human | Cytokines and cytokine receptors | 423 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HCD-M02 | Human | CDs | 426 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HCV-M02 | Human | SARS-CoV-2 host interaction genes | 332 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HDG-M02 | Human | Drug target genes | 8611 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HEP-M02 | Human | Epigenetics | 656 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HG-M02 | Human | Genome collection | 12841 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HGR-M02 | Human | GPCRs | 875 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HHI-M02 | Human | Histones | 73 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HHM-M02 | Human | Histone modification enzymes | 107 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HIC-M02 | Human | Ion channels | 448 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HII-M02 | Human | Innate immune proteins | 644 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HIO-M02 NEW! | Human | Immuno-oncology | 6592 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HKI-M02 | Human | Kinases | 666 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HMP-M02 | Human | Membrane bound proteins | 7294 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HNH-M02 | Human | Nuclear hormone receptors | 73 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HPH-M02 | Human | Phosphatases | 288 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HPI-M02 | Human | Protease inhibitors | 154 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HPR-M02 | Human | Protease | 596 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HSP-M02 | Human | Secretory proteins | 2334 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| OC-HTF-M02 | Human | Transcription factors | 2412 | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
| Custom order | Various | Custom-selected | Various | pReceiver-M02 Non-viral |
Expression-ready Non-viral |
Inquire |
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- 作者
- 内容
- 询问日期
文献和实验- Zheng, Y., et al. (2023). Chronic psychological stress promotes breast cancer pre-metastatic niche formation by mobilizing splenic MDSCs via TAM/CXCL1 signaling. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02696-z [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Dual Luminescence Assay Kit; OMICSLINK™ Lentiviral ORF vector; polybrene reagent]
- Quintanal-Villalonga, A., et al. (2023). Exportin 1 inhibition prevents neuroendocrine transformation through SOX2 down-regulation in lung and prostate cancers. SCIENCE TRANSLATIONAL MEDICINE doi: 10.1126/scitranslmed.adf7006 [GLuc-ON™ promoter-reporter clone; OMICSLINK™ ORF expression control clones]
- Bergholz, J.S., et al. (2023). PI3Kβ controls immune evasion in PTEN-deficient breast tumours. Nature doi: 10.1038/s41586-023-05940-w [OMICSLINK™ mouse Pik3cb ORF expression clone]
- Sanmarco, L.M., et al. (2023). Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature doi: 10.1038/s41586-023-06409-6 [OMICSLINK™ NDUFA4L2 ORF expression clone and empty control vector; Secrete-Pair™ Gaussia Luciferase Assay Kit]
- Ke, H., et al. (2023). Landscape of pathogenic mutations in premature ovarian insufficiency. Nature Medicine doi: 10.1038/s41591-022-02194-3 [OMICSLINK™ empty vector; Secrete-Pair™ dual luminescence kit]
- Jiang, L., et al. (2023). Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress. SCIENCE ADVANCES doi: 10.1126/sciadv.add9789 [OMICSLINK™ NEUROG2 Lentiviral ORF expression clone]
- Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [OMICSLINK™ empty vector; GLuc-ON™ promoter reporter clone; Lenti-Pac™ HIV Expression Packing Kit; Secrete-Pair™ Dual Luminescence Assay Kit]
- Yan, B., et al. (2023). The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression. Exp Mol Med doi: 10.1038/s12276-023-00997-z [OmicsLink™ Expression Clone vectors; OMICSLINK™ shRNA expression clone]
- Watson, D.C., et al. (2023). GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity. Nat Cancer doi: 10.1038/s43018-023-00556-5 [OMICSLINK™ empty vector]
- Lee, J.H., et al. (2023). The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner ear. Res Sq doi: 10.21203/rs.3.rs-2287052/v1 [OMICSLINK™ Pdch15, Tmie , Lfhpl5, Tmc1 and Cib2 ORF expression clone]
- Li, B., et al. (2023). Cell networks in the mouse liver during partial hepatectomy. bioRxiv doi: 10.1101/2023.07.16.549116 [OMICSLINK™ Fstl1 expression clone]
- Wasser, C., et al. (2023). Apoer2-ICD-dependent regulation of hippocampal ribosome mRNA loading. Res Sq doi: 10.21203/rs.3.rs-3040567/v1 [Gluc + SEAP reporter clone pEZX-GA01]
- Ankenbauer, K.E., et al. (2023). Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics. bioRxiv doi: 10.1101/2023.06.03.543566 [OMICSLINK™ ST6GAL1 ORF expression clone]
- Sun, Y., et al. (2023). ZDHHC2-Mediated AGK Palmitoylation Activates AKT–mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma. Cancer Res doi: 10.1158/0008-5472.CAN-22-3105 [OMICSLINK™ ZDHHC2 and AGK ORF expression clone]
- He, B., et al. (2023). IL-13/IL-13RA2 signaling promotes colorectal cancer stem cell tumorigenesis by inducing ubiquitinated degradation of p53. Genes Dis doi: 10.1016/j.gendis.2023.01.027 [OMICSLINK™ IL-13RA2 and p53 ORF expression clone]
- Naulé, L., et al. (2023). MKRN3 inhibits puberty onset via interaction with IGF2BP1 and regulation of hypothalamic plasticity. JCI Insight doi: 10.1172/jci.insight.164178 [OMICSLINK™ MKRN3 ORF expression clone]
- Yuan, Z-L., et al. (2023). Inhibition of ERK downregulates autophagy via mitigating mitochondrial fragmentation to protect SH-SY5Y cells from OGD/R injury. Cell Commun Signal doi: 10.1186/s12964-023-01211-3 [OMICSLINK™ Mfn2 and Drp1 ORF expression clone]
- Yang, Y-C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [OMICSLINK™ NEDD4L ORF expression clone; Secrete-Pair™ Gaussia Luciferase Assay kit]
- Peng, S., et al. (2023). PHF8-GLUL axis in lipid deposition and tumor growth of clear cell renal cell carcinoma. Sci Adv doi: 10.1126/sciadv.adf3566 [OMICSLINK™ TEAD1 ORF expression clone]
- Song, C., et al. (2023). RA-induced prominence-specific response resulted in distinctive regulation of Wnt and osteogenesis. Life Sci Alliance doi: 10.26508/lsa.202302013 [OMICSLINK™ RARE ORF expression clone]
- Hu, X., et al. (2023). Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2‐positive gastric cancer. Cancer Commun (Lond) doi: 10.1002/cac2.12459 [OMICSLINK™ CDC42 ORF expression clone]
个内源性表达的细胞周期基因。为此我们使用LightCycler® 480实时荧光定量PCR仪和RealTime ready细胞周期检测预制多孔板。用特异抗体和流式细胞术测定天冬氨酸特异性半胱氨酸蛋白酶-3的激活。在这个分析中,我们发现C24ORF17和BARD1基因下调后,天冬氨酸特异性半胱氨酸蛋白酶-3的激活增加了。为了确定编码蛋白在凋亡诱导中的作用,在本研究中我们使用RealTime ready凋亡检测预制多孔板和LightCycler® 480仪器,通过qRT-PCR分析了当通过RNAi而表达
连接(可设计包含酶切位点的引物,PCR后酶切连接或直接进行平端连接),这样5000bp片段就得到了。 本公司提供40000多条人、小鼠、斑马鱼ORF/cDNA基因克隆,80多种表达载体,50多种融合标签,另外接受基因克隆服务,有意联系:15360479798,QQ:1598921096.(公司在广州的^-^) 版主dnazyme留言: 凡是有广告或者广告嫌疑的,即使有帮助也不加分。 本文由丁香园论坛提供,想了解更多有用
相关专题 DNA微阵列基因表达数据分析 本文利用Matlab及其生物信息学 工具箱提供的函数识别差异表达基因并利用基因本体论确定差异表达基因的生物学功能。 引言 包含寡核苷酸或cDNA 探针的微阵列可用来比较基因组尺度的基因表达谱,微阵列试验的重要目的在于确定不同条件下,如两种不同的肿瘤类型,是否存在统计显著的基因表达量的变化进而确定差异表达基因的生物学功能。 本文利用一个公共数据集来说明计算过程
技术资料








