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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Mouse apolipoprotein E (Apo-E) ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
400 0.157 0.159 0.158
200 0.249 0.269 0.259
100 0.432 0.441 0.437
50 0.643 0.672 0.658
25 0.955 0.984 0.970
12.5 1.199 1.172 1.186
6.25 1.568 1.572 1.570
0 2.573 2.591 2.582
Mouse apolipoprotein E (Apo-E) ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Mouse apolipoprotein E (Apo-E) ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:100 Average % 99
Range % 96-101
1:200 Average % 85
Range % 81-89
1:400 Average % 100
Range % 97-103
1:800 Average % 83
Range % 80-86
Mouse apolipoprotein E (Apo-E) ELISA Kit
回收率:The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-100
EDTA plasma (n=4) 99 95-104
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文献和实验Genotyping of Apolipoprotein E
Apolipoprotein E (apo E) is a 299-amino acid plasma protein involved in cholesterol transport and is found in chylomicrons, very low density lipopro-tein, intermediate-density lipoprotein, and high-density lipoprotein (1 ,1 ).
Apolipoprotein E Expression and Purification
Since the discovery of the association of apolipoprotein E (apoE) 4 with Alzheimer’s disease 17 years ago, numerous in vitro experiments with the apoE isoforms (apoE2, apoE3, and apoE4) have been performed to try to understand the basis
Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
Hepatic lipase (HL) is necessary for efficient clearance of triglyceride-rich lipoproteins from plasma (1 ). In humans, HL deficiency leads to the accumulation of triglyceride-rich intermediate density lipoproteins (IDL), LDL, and large HDL
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