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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human Adenomatous polyposis coli protein(APC) ELISA kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.770 2.713 2.742 2.628
1500 1.574 1.555 1.565 1.451
750 0.774 0.762 0.768 0.654
375 0.499 0.521 0.510 0.396
187.5 0.292 0.307 0.300 0.186
94 0.223 0.231 0.227 0.113
47 0.189 0.184 0.187 0.073
0 0.115 0.113 0.114
Human Adenomatous polyposis coli protein(APC) ELISA kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human Adenomatous polyposis coli protein(APC) ELISA kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 89
Range % 85-92
1:2 Average % 101
Range % 97-104
1:4 Average % 93
Range % 90-95
1:8 Average % 90
Range % 86-94
Human Adenomatous polyposis coli protein(APC) ELISA kit
回收率:The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-94
EDTA plasma (n=4) 87 84-92
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文献和实验Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis ColiGene
Most germline mutations of the adenomatous polyposis coli (APC ) tumor suppressor gene result in a classic inherited cancer syndrome called familial adenomatous polyposis (FAP). FAP is characterized by thousands of colonic polyps
酶活性的DNA聚合酶，原来通常使用的是Taq DNA聚合酶，缺少校正功能。在这儿我们展示将长PCR技术应用到5＇RACE系统中，来分离5.5kb长的人结节性脑硬化II（tuberous sclerosis II，TSC-2）mRNA和8.9kb长的腺瘤性结肠息肉（adenomatous polyposis coli，APC）mRNA的5＇末端，扩增的5＇末端片段要比单独用Taq DNA聚合酶获得的片段长。方法使用5＇RACE系统版本2 （Cat. No. 18374-058）来扩增TSC-2和APC
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells Methods in Molecular Biology v468. chapter 6 Abstract The adenomatous polyposis coli (APC) tumour suppressor gene is mutated
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