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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human TNF α converting enzyme,TACE ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 1.931 1.872 1.902 1.770
2000 1.648 1.596 1.622 1.490
1000 1.335 1.304 1.320 1.188
500 1.082 1.012 1.047 0.915
250 0.643 0.609 0.626 0.494
125 0.409 0.379 0.394 0.262
62.5 0.274 0.257 0.266 0.134
0 0.135 0.128 0.132
Human TNF α converting enzyme,TACE ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human TNF α converting enzyme,TACE ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human TACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 84
Range % 80-90
1:2 Average % 97
Range % 92-103
1:4 Average % 92
Range % 86-99
1:8 Average % 93
Range % 85-98
Human TNF α converting enzyme,TACE ELISA Kit
回收率:The recovery of human TACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-100
EDTA plasma (n=4) 97 90-103
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
) abs510003-96T Human IL-6 ELISA Kit abs510006-96T Human TNF-α ELISA Kit abs510002-96T Human IL-1β ELISA Kit abs510004-96T Human IL-8/CXCL8 ELISA Kit abs520003-96T Mouse IL-4 ELISA Kit abs520006-96T Mouse IL-12 p70 ELISA Kit abs520007-96
酸氧化；目前均以临床前研究为主。 Starter品牌Onestep Elisa Kit，助力细胞因子检测 一小时实验，一次孵育，一次洗板！ 工作量减半！效率翻倍！ 优宁维ELISA全方位满足不同实验需求,助力细胞因子检测 货号 名称 S0C3024-HL2 Human TNF-α Onestep ELISA Kit S0C3139 Mouse TNF-α (serum&plasma) OneStep ELISA Kit S0C3013 Human IL-1β OneStep
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