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文献和实验NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
of both the transformation efficiency and gene expression (1 ). The most commonly used selectable marker is the gene from Transposon 5 (Tn5) of Escherichia coli K12 (aphA2) (2 ), which encodes aminoglycoside 3-phosphotransferase II (APH [3] II, Chemical Abstracts Registry
ProteinProtein Interaction Techniques: Dissect PCP Signaling in Xenopus
. Xenopus embryo has been established as an excellent model system to dissect PCP signaling in vertebrates because morphogenetic cell behaviors including CE can easily be monitored in vivo. Xenopus Paraxial protocadherin (xPAPC) is a transmembrane protein
基因。 通过对200个荷兰哮喘家系进行遗传相关分析和原位克隆技术研究,发现了第5号染色体的钙黏素(cadherin)超家族成员原钙黏素(protocadherin-1,PCDHl)基因与气道高反应性相关。PCDHl是钙调蛋白家族中的一员,主要在肺组织中表达,尤其是支气管上皮细胞。染色体2q33上某些SNP位点与荷兰哮喘人群的总血浆IgE水平,BHR间存在明显的关联性。
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