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- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Aldolase B, Fructose Bisphosphate (ALDOB)
- 库存:
1000
- 供应商:
上海信裕
果糖二磷酸醛缩酶B(ALDOB)重组蛋白
Recombinant Aldolase B, Fructose Bisphosphate (ALDOB)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy319Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | >95% |
| UOM | 50ug |
| Predicted Molecular Mass | 40.6kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
果糖二磷酸醛缩酶B(ALDOB)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
IA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKE KGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRA VLRIADQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VIPDGDHDLE HCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF SYGRALQASA LAAWGGKAAN KEATQEAFMK RAMANCQAAK GQYVHTGSSG AASTQSLFTA CYTY
IA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKE KGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRA VLRIADQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VIPDGDHDLE HCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF SYGRALQASA LAAWGGKAAN KEATQEAFMK RAMANCQAAK GQYVHTGSSG AASTQSLFTA CYTY
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
果糖二磷酸醛缩酶B(ALDOB)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
果糖二磷酸醛缩酶B(ALDOB)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
果糖二磷酸醛缩酶B(ALDOB)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
类器官类型或特定细胞类型形成组装体来提升。图像代表小鼠肝脏组装体,PDGFRα–H2B–GFP标记门脉成纤维细胞核(绿色),核tdTom标记导管细胞核(品红色),肝细胞核显示为蓝色,鬼笔环肽染色显示细胞骨架(白色)。 c:通过生物打印、水凝胶模具或脱细胞支架生成2.5维类组织结构,实现结构复杂性。下方图像代表用于形状引导的定制微流控芯片(左图),以及在带有激光雕刻管状支架的微流控芯片上培养的组织干细胞来源人类成人肠道类器官(右图)。细胞染色显示E-钙黏蛋白(E-cad)与醛缩酶B(AldoB)。 d
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果糖二磷酸醛缩酶B(ALDOB)重组蛋白
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