- 询价
- PEPROTECH
- 中国/美国
- xy-589Hu01
- 2025年07月12日
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Heme Oxygenase 2, Decycling (HO2)
- 库存：
1000
- 供应商：
上海信裕
血红素氧合酶2(HO2)重组蛋白
Recombinant Heme Oxygenase 2, Decycling (HO2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy589Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.8kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
血红素氧合酶2(HO2)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTALYFTYSA LEEEMERNKD HPAFAPLYFP MELHRKEALT KDMEYFFGEN WEEQVQCPKA AQKYVERIHY IGQNEPELLV AHAYTRYMGD LSGGQVLKKV AQRALKLPST GEGTQFYLFE NVDNAQQFKQ LYRARMNALD LNMKTKERIV EEANKAFEYN MQIFNELDQA GSTLARETLE DGFPVHDGKG DMRKCPFYAA EQDKGALEGS SCPFRTAMAV LRKPSLQFIL AAGVAL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTALYFTYSA LEEEMERNKD HPAFAPLYFP MELHRKEALT KDMEYFFGEN WEEQVQCPKA AQKYVERIHY IGQNEPELLV AHAYTRYMGD LSGGQVLKKV AQRALKLPST GEGTQFYLFE NVDNAQQFKQ LYRARMNALD LNMKTKERIV EEANKAFEYN MQIFNELDQA GSTLARETLE DGFPVHDGKG DMRKCPFYAA EQDKGALEGS SCPFRTAMAV LRKPSLQFIL AAGVAL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
血红素氧合酶2(HO2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血红素氧合酶2(HO2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血红素氧合酶2(HO2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
生成素(EPO)转铁蛋白、血红素加氧酶-1等的基因，它们都有一个共同的顺式作用元件(CGTG)，有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合，激活靶基因的转录，在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 4．提高蛋白表达产量的措施 哺乳动物细胞对培养环境十分敏感，营养和生长因子缺乏、缺氧、病毒感染、毒性代谢
。这些启动子在诱导前后活性可相差4个数量级。另外，在哺乳动物细胞中已发现存在大量在低氧环境中可诱导转录的基因，如编码红细胞生成素(EPO)转铁蛋 白、血红素加氧酶-1等的基因，它们都有一个共同的顺式作用元件(CGTG )，有利于在5’或3’侧翼区的低氧诱导低氧诱导作用因子-1(HIF-1)和低氧反应增强子(HRE)结合，激活靶基因的转录，在低氧浓度下可使重组蛋白大量表达。据报道红细胞生成素启动子在 1%氧浓度比在21%氧浓度下活陛增强100倍。这又是一种新的提高表达量的作用机制。 2、宿主
技术资料暂无技术资料 索取技术资料
血红素氧合酶2(HO2)重组蛋白
询价
