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- 2025年07月10日
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- 库存：
10
- 供应商：
上海恪敏生物科技有限公司
- 规格：
20preps
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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
"Glassing Out" DNA Using the Geneclean Kit
a fresh razor blade and place into a clean Eppendorf tube. Use long-wave UV light for as short a time as possible to visualize the DNA. 2. Determine the approximate volume of the gel slice by weight. If the slice weighs more than 0.4 g
3 times with 500μl wash solution opellet silica once more to remove any residual wash solution oair dry to remove any residual EtOH 3.Elute DNA from silica oresuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer
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