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- 供应商：
上海恪敏生物科技有限公司
- 规格：
25preps
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文献和实验Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
Materials: see Solutions for Recipes TE buffer 10% (w/v) sodium dodecyl sulfate (SDS ) 20 mg/ml proteinase K
SOUTHERN BLOTTING OF GENOMIC DNA
a clean blade to notch one side of the membrane to help orient it to the gel (I make a notch on the top of the gel by lane #1).Label the membrane using a pencil. • Carefully remove the membrane and place,DNA side up,in a dish containing ca.200 ml of 3X
Analys of Genomic DNA by Southe
from vector.) (To ensure homogeneous dispersion of the genomic DNA: (a) allow the DNA to stand at 4℃ for several hours after dilution and addition of 10 x restriction enzyme buffer, (b) gently stir the DNA solution from time to time, (c) after adding
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