- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-468Ra01
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Decay Accelerating Factor (DAF)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy468Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 14.7kDa |
| Concentration | 10.1 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSG SEF- PHESTLI NFPSTRAPLS HKPTTVKVPG TRVKPTSHKP TEVKVPATQH VPVSKTTVRH PTRTSKDRGE SNSGGDHFIY GFVAVIVTID SIILIKTLWW TILSSNRSSD LQGKKKRENV PE
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
衰变加速因子(DAF)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验网络 三、促衰变因子（CD55） 促衰变因子（decay accelerating factor,DAF）是Nicholson-Weller等（1981）用正丁醇提取后，再以层析法从人和豚鼠红细胞基质中纯化的一种膜蛋白。因其具有促进C3转化酶衷变的活性故名。经在还原条件下做SDS-PAGE并以过碘酸
CD55 分子 CD55 常用单克隆抗体或代号： 143- 30,BRIC110,BRIC123;(DAF) 主要表达细胞： 广泛 [NL] 分子质量(kDa)和结构： gp70(GPI连接) 功 能： 衰变加速因子,调节补体活化 CD55 Aka decay accelerating factor Binds C3bBb (alternative pathway
b，由此抑制补体各激活途径。C4结合蛋白（C4bbindingprotein，C4bp）抑制C4b与C2的结合，辅助I因子裂解C4b.当这些因子缺陷时可出现临床相应病症。如遗传性C1抑制分子缺乏可发生遗传性血管神经性水肿. 2.细胞膜上的调节因子 抑制C3转化酶组装并加速其解离，协助I因子裂解C3b和C4b.膜辅助蛋白（MCP）辅助I因子裂解C3b和C4b.衰变加速因子（DAF）与C4b结合，抑制C3转化酶形成并促进其分解。同源限制因子（HRF）又称C8结合蛋白（C8bp），能与C8结合
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