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- xy-U233Hu01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Recombinant Von Willebrand Factor A Domain Containing Protein 5B2 (vWA5B2)
- 库存：
1000
- 供应商：
上海信裕
含血管性血友病因子A域结合蛋白5B2(vWA5B2)重组蛋白
Recombinant Von Willebrand Factor A Domain Containing Protein 5B2 (vWA5B2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy-U233Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 22.7kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu354~Ile527 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
含血管性血友病因子A域结合蛋白5B2(vWA5B2)重组蛋白 SEQUENCES
The sequence of the target protein is listed below.
ELLFLLD SSSVAHKDAI VLAVKSLPPQ TLINLAVFGT LVQPLFPESR PCSDDAVQLI CESIETLQVP SGPPDVLAAL DWAVGQPQHR AYPRQLFLLT AASPMAATTH RTLELMRWHR GTARCFSFGL GPTCHQLLQG LSALSRGQAY FLRPGQRLQP MLVQALRKAL EPALSDI
ELLFLLD SSSVAHKDAI VLAVKSLPPQ TLINLAVFGT LVQPLFPESR PCSDDAVQLI CESIETLQVP SGPPDVLAAL DWAVGQPQHR AYPRQLFLLT AASPMAATTH RTLELMRWHR GTARCFSFGL GPTCHQLLQG LSALSRGQAY FLRPGQRLQP MLVQALRKAL EPALSDI
USAGE
Reconstitute in sterile ddH2O.
含血管性血友病因子A域结合蛋白5B2(vWA5B2)重组蛋白 STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
含血管性血友病因子A域结合蛋白5B2(vWA5B2)重组蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
物种蛋白同源性比对。如果比对结果，证明同源性比较高的，也可以选择。 （3）分析样本中的蛋白质的结构性质 了解样本蛋白的结构性质有助于选择最合适的抗体，至少两方面因素需要考虑： 待测样本蛋白的结构域：抗体是由各种不同免疫原免疫宿主而制备得来，其中的免疫原包括：全长蛋白、蛋白片断、多肽、全有机体（如：细菌）或细胞，抗体说明书一般都有免疫原的描述，如果打算检测的是蛋白片断或一种特殊的同型物或蛋白全长的某一区域，则必须选择用含此片段域的免疫原制备出的抗体。如果打算用FCM
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含血管性血友病因子A域结合蛋白5B2(vWA5B2)重组蛋白
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