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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Interleukin 13 Receptor Alpha 1 (IL13Ra1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | xy641Mu02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 19.5kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSGS-IMVKDNAGKI RPSCKIVSLT SYVKPDPPHI KHLLLKNGAL LVQWKNPQNF RSRCLTYEVE VNNTQTDRHN ILEVEEDKCQ NSESDRNMEG TSCFQLPGVL ADAVYTVRVR VKTNKLCFDD NKLWSDWSEA QSIGKEQNST FYTTMLLTIP VFVAVAVIIL
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
白介素13受体α1(IL13Rα1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验内源性FSTL1 通过激活钠‐钾泵引起膜超极化调节痛觉信息传递
的重组FSTL1 蛋白孵育培养的大鼠DRG 神经元,BS3 交联与细胞膜上受体结合的FSTL1,裂解细胞。细胞裂解物做Western blotting,用抗Myc 的抗体进行免疫共沉淀(IP),在140 kD 处发现阳性条带。于FSTL1 约40 kD,因此推断FSTL1 结合了约100 kD 的蛋白。相同的方法富集交联复合物,用质谱分析发现结合蛋白可能为NKA 的α1 亚基。放免实验和co‐IP 实验证明了与FSTL1 结合的蛋白正是NKA 的α1 亚基。 由于FSTL1 能通过清亮小泡
性。 3.IFN-α/β体受 一般认为,IFN-α和IFN-β结合相同的受体,IFT-α/βR基因定位于21号染色体,受体的亲和力Kd在10-9~10-10M之间,受体胞膜外结构属细胞因子受体中干扰素受体家族。IFN-α/β受体分布相当广泛,包括单核细胞、巨噬细胞、多形核白细胞、B细胞、T细胞、血小板、上皮细胞、内皮细胞和肿瘤细胞等。 4.IFN-α/β的生物学作用 (1)抗病毒作用:IFN-α/β具有广谱的抗病毒作用,其作用机理是:①通过抑制某些病毒的吸附(如VSV
32) 40 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcγRⅢ(CD16) 50~70 - 2 - 1q23-q24 IgG Fc低亲和力受体 FcεRⅠα链 25
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