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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Hedgehog Homolog, Indian (IHH)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy116Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
印度刺猬因子(IHH)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Tubulin55kDa胞浆和全细胞VCDA1/Porin31kDa线粒体COXIV16kDa线粒体Lamin B116kDa细胞核(不适于去除核膜的样本)TBP38kDa胞浆和全细胞转膜PVDF 膜NC 膜尼龙膜灵敏度和分辨率高高高背景低低较高蛋白结合能力100-200μg/cm2 (适用于SDS 存在时与蛋白结合)80-100μg/cm2大于400μg/cm2机械强度强干的膜易脆软而结实溶剂抗性强差差浸润100% 甲醛缓冲液缓冲液适用染色方法胶体金、丽春红、酰胺黑、印度墨汁胶体金、丽春
》(PNAS)。该论文在线之际,受到国外Scientist,Nature news,The Australian,Thomson Reuters, Fox News, Agence France Presse (AFP法新社) 等美国、英国、俄罗斯、德国、巴西、印度各专业杂志及媒体的广泛关注和报道。 该研究表明由转基因水稻种子生产的重组人血清白蛋白(OsrHSA)在生理生化性质、物理结构,生物学功能、免疫原性与血浆来源的人血清白蛋白一致;并建 立了大规模生产重组人血清白蛋白的生产工艺,获得了高纯度和高
印迹前常需要通过免疫沉淀,亚细胞分离等手段富集。 Western实验步骤 一、样品制备 对于Western Blotting实验而言,样品处理是关键步骤之一,获得的蛋白样品必须均质、可溶,并解离成单个多肽亚基,且尽量减少相互间的聚集,使其最终仅依赖本身的分子量大小进行分离。当目标蛋白的含量很低时,需要选取目标蛋白含量较高的组织或细胞器(如核抽提物),或者通过免疫沉淀等方式进行富集。通常样品有重组蛋白、细胞和组织等。 1. 样品收集 1)培养
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