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- PEPROTECH
- 中国/美国
- xy-828Hu01
- 2026年01月07日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Tripeptidyl Peptidase I (TPP1)
- 库存：
1000
- 供应商：
上海信裕
三肽基肽酶Ⅰ(TPP1)重组蛋白
Recombinant Tripeptidyl Peptidase I (TPP1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy828Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 38.2kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
三肽基肽酶Ⅰ(TPP1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MRLFGGNFAH QASVARVVGQ QGRGRAGIEA SLDVQYLMSA GANISTWVYS SPGRHEGQEP FLQWLMLLSN ESALPHVHTV SYGDDEDSLS SAYIQRVNTE LMKAAARGLT LLFASGDSGA GCWSVSGRHQ FRPTFPASSP YVTTVGGTSF QEPFLITNEI VDYISGGGFS NVFPRPSYQE EAVTKFLSSS PHLPPSSYFN ASGRAYPDVA AGYWVVS NRVPIPWVSG TSASTPVFGG ILSLINEHRI LSGRPPLGFL NPRLYQQHGA GLFDVTRGCH ESCLDEEVEG QGFCSGPGWD PVTGWGTPNF PALLKTLLNP
MRLFGGNFAH QASVARVVGQ QGRGRAGIEA SLDVQYLMSA GANISTWVYS SPGRHEGQEP FLQWLMLLSN ESALPHVHTV SYGDDEDSLS SAYIQRVNTE LMKAAARGLT LLFASGDSGA GCWSVSGRHQ FRPTFPASSP YVTTVGGTSF QEPFLITNEI VDYISGGGFS NVFPRPSYQE EAVTKFLSSS PHLPPSSYFN ASGRAYPDVA AGYWVVS NRVPIPWVSG TSASTPVFGG ILSLINEHRI LSGRPPLGFL NPRLYQQHGA GLFDVTRGCH ESCLDEEVEG QGFCSGPGWD PVTGWGTPNF PALLKTLLNP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
三肽基肽酶Ⅰ(TPP1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
三肽基肽酶Ⅰ(TPP1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
三肽基肽酶Ⅰ(TPP1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
因子的Xa系列。 TAGZyme系统的主要组成部分是一种外切蛋白酶――二肽氨基肽酶Ⅰ（dipeptidyl aminopeptidase I，DAPase），如果你表达的外源蛋白不含有外切酶识别的停顿位点，那么你还需要联合使用谷氨酸循环转移酶（glutamine cyclotransferase，Qcyclase）和焦谷氨酸氨基肽酶（pyroglutamyl aminopeptidase，pGAPase）。这些酶都经过重组，可以通过Ni-NTA填料除去。应用TAGZyme系统的反应是十分灵敏的，不会
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三肽基肽酶Ⅰ(TPP1)重组蛋白
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