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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Bcl2 Antagonist/Killer 1 (BAK1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | xy324Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Bcl2拮抗/杀伤因子1(BAK1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验(Tumornecrosisfactor,TNF)根据其产生来源和结构不同,可分为TNF-α和TNF-β两类,前者由单核-巨噬细胞产生,后者由活化T细胞产生,又名淋巴毒素。TNF除具有杀伤肿瘤细胞外,还有免疫调节、参与发热和炎症的发生。 5,转化生长因子-β家族(Transforminggrowthfactor-βfamily,TGF-βfamily)主要包括TGF-β1、TGF-β2、TGF-β3、TGFβ1β2以及骨形成蛋白(BMP)等。 6.趋化因子家族(Chemokinefamily)包括两个亚族:(1)C-X-C
浸润淋巴细胞(TIL) ● Human and murine Lymphokine Activated Killer (LAK) cells 人和鼠淋巴因子激活杀伤细胞(LAK) ● Human and murine hematopoietic stem cells (colony formation and proliferation) 人和鼠造血干细胞(克隆形成和增殖) X-VIVO产品的应用 ◆TIL的增殖 ◆PBL的增殖 ◆干细
结合,无论标签位于蛋白的N端、C端或是内部。需要注意的是,在非变性条件下纯化时,His标签必须充分暴露在蛋白表面以利于充分的纯化。 6、是否可以把His标签从表达蛋白上切割掉? 是的。QIAGEN提供一种载体,称为pQE-30 Xa, 与pQE-30相似,但在His标签与多克隆位点之间有编码因子Xa蛋白酶识别位点的序列。因子Xa蛋白酶可以在蛋白酶识别位点的精氨酸之后进行切割。经过因子Xa蛋白酶处理可以得到不含组氨酸的重组蛋白。 7、哪一种抗His抗体最灵敏? Penta His和Tetra
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