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- PEPROTECH
- 中国/美国
- xy-801Hu01
- 2026年03月30日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Prekallikrein (PK)
- 库存：
1000
- 供应商：
上海信裕
激肽释放酶原(PK)重组蛋白
Recombinant Prekallikrein (PK)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy801Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 27.2kDa |
| Concentration | 8.2 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
激肽释放酶原(PK)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TFHKA EYRNNCLLKY SPGGTPTAIK VLSNVESGFS LKPCALSEIG CHMNIFQHLA FSDVDVARVL TPDAFVCRTI CTYHPNCLFF TFYTNVWKIE SQRNVCLLKT SESGTPSSST PQENTISGYS LLTCKRTLPE PCHSKIYPGV DFGGEELNVT FVKGVNVCQE TCTKMIRCQF FTYSLLPEDC KEEKCKCFLR LSMDGSPTRI AYGT
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TFHKA EYRNNCLLKY SPGGTPTAIK VLSNVESGFS LKPCALSEIG CHMNIFQHLA FSDVDVARVL TPDAFVCRTI CTYHPNCLFF TFYTNVWKIE SQRNVCLLKT SESGTPSSST PQENTISGYS LLTCKRTLPE PCHSKIYPGV DFGGEELNVT FVKGVNVCQE TCTKMIRCQF FTYSLLPEDC KEEKCKCFLR LSMDGSPTRI AYGT
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
激肽释放酶原(PK)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
激肽释放酶原(PK)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
激肽释放酶原(PK)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
接触凝血因子包括经典FⅫ、FⅪ和激肽系统的激肽释放酶原（PK）医学教育|网搜集整理、高分子量激肽原（HMWK）。它们的共同特点是通过接触反应启动内源凝血途径，并与激肽、纤溶和补体等系统相联系。
凝血因子目前包括14个，除FⅢ存在于全身组织中，其余均存在于血浆中。根据理化性质分为四组。 （1）依赖维生素K凝血因子： 包括FⅡ、FⅦ、FⅨ和FⅩ，其共同特点是在各自分子结构的氨基末端含有数量不等的γ-羧基谷氨酸残基，在肝合成中必须依赖维生素K.依赖维生素K凝血因子（依K因子）通过γ-羧基谷氨酸与Ca2+结合，再与磷脂结合，这是依K因子参与凝血反应的基础。 （2）接触凝血因子： 包括经典FⅫ、FⅪ和激肽系统的激肽释放酶原（PK）、高分子量激肽原（HMWK
功能与免疫功能相配合，有效的保护机体，减少创伤带来的损害。 图3-7表面激活也血液各种防卫功能关系示意图 PK：前激肽释放酶 Pn：纤溶酶原 K：激肽释放酶 Pn：纤溶酶 HK：高分子激肽原 CI：补体 Kn：激肽 Ⅻ与Ⅻa，因子Ⅻ及其激活型
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激肽释放酶原(PK)重组蛋白
¥1 - 3980
