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- PEPROTECH
- 中国/美国
- xy-585Hu01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Glypican 2 (GPC2)
- 库存：
1000
- 供应商：
上海信裕
磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白
Recombinant Glypican 2 (GPC2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy585Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
完全活化成为效应T细胞。在正常生理情况下，共刺激分子与共抑制分子之间的平衡，使T细胞的免疫效应保持适当的深度、广度，从而最大程度减少对于周围正常组织的损伤，维持对自身组织的耐受、避免自身免疫反应。然而，肿瘤细胞可异常上调共抑制分子PD-1、PD-L1的含量。PD-L1与PD-1结合后，PD-1胞质区ITSM结构域中的酪氨酸发生磷酸化，募集SHP-2磷酸酶，使下游的Syk和磷脂酰肌醇3激酶(PI3K)发生去磷酸化，从而传递抑制性信号抑制淋巴细胞的功能及细胞因子的释放，打破共刺激分子与共抑制分子之间
farnesylcysteine|法尼半胱氨酸 farnesylpyrophosphate|法尼焦磷酸，焦磷酸法尼酯 Farr technique|Farr技术，法尔技术[测定抗体绝对量的放射免疫技术] Farwestern blotting|Farwestern印迹法，蛋白质检测（的）蛋白质印迹法[例如用标记的激酶或重组蛋白 来检测印迹膜上与之作用的蛋白] fasciclin|成束蛋白[参与轴突的成束]
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磷脂酰肌醇蛋白聚糖2(GPC2)重组蛋白
¥1 - 3980
