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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Monocyte To Macrophage Differentiation Associated Protein (MMA)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | xy584Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
单核细胞巨噬细胞分化关联蛋白(MMA)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验毒性作用。EST12 蛋白导致小鼠腹腔巨噬细胞(图 1A 和 B)、人单核细胞 THP-1(图 1C)和小鼠骨髓来源巨噬细胞(BMDMs)死亡(图 1D)。结果表明,EST12 对巨噬细胞具有较强的毒性。 图 1 巨噬细胞焦亡诱导蛋白 EST12 的鉴定 A-D:分别用 EST12 和 Rv1577 处理小鼠腹腔巨噬细胞(A、B)、PMA 分化的 THP1 细胞(C)、小鼠骨髓巨噬细胞 BMDMS(D),采用 LDH 释放法分析。E:WB 检测 2μM EST12 或 RV1577 处理巨噬细胞
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