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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the N/C-term region of human protein.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H, M, R
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
HRP AQP-2
- 抗体英文名:
HRP AQP-2 Antibody
- 抗体名:
HRP AQP-2 Antibody
- 规格:
0.1 mg
HRP AQP-2 Antibody产品详细介绍:
| 产品名称: | HRP AQP-2 Antibody |
| 说明书: | http://abbiotec.com/antibodies/hrp-aqp-2-antibody【参考图片】 |
| 货号: | 250697 |
| 规格: | 0.1 mg |
| 产品描述: | |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the N/C-term region of human protein. |
| 适用物种: | H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:200-1:500; IHC: 1:200-1:500 |
| 别名: | Aquaporin-2; AQP-2; Aquaporin-CD; AQP-CD; Water channel protein for renal collecting duct: ADH water channel; Collecting duct water channel protein; AQP2 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
1 ) ,再与 HRP 标记的 AQP1 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的水通道蛋白 1 ( AQP1 ) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人水通道蛋白 1 ( AQP1 ) 浓度。 试剂盒组成 : 试剂盒组成
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