- ￥3000 - 3999
- Abbiotec
- 美国
- 250444
- 2025年07月10日
- E, WB
- Mouse
- Ca
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant von Willebrandt domain of caiman factor B
- 亚型：
Mouse IgG2a/k
- 形态：
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件：
Store at 4°C.
- 克隆性：
单克隆抗体
- 标记物：
见下方详细说明
- 适应物种：
Ca
- 宿主：
Mouse
- 应用范围：
E, WB
- 浓度：
见详细说明信息
- 靶点：
Factor B Caiman (11-B28)
- 抗体英文名：
Factor B Caiman (11-B28) Antibody
- 抗体名：
Factor B Caiman (11-B28) Antibody
- 规格：
0.2 mg
Factor B Caiman (11-B28) Antibody产品详细介绍：
| 产品名称： | Factor B Caiman (11-B28) Antibody |
| 说明书： | http://abbiotec.com/antibodies/factor-b%2C-caiman-%2811-b28%29-antibody【参考图片】 |
| 货号： | 250444 |
| 规格： | 0.2 mg |
| 产品描述： | Complement factor B plays an imortant role in the alternative complement activation pathway by complexing with C3b to create the active C3 convertase. The alternative activation pathway was the first to evolve, thus the components involved are very similar in physological different species. The caiman complement factor B is similar to the mammalian counterpart and consists of a single polypeptide chain of approximately 90 kDa which upon activation is cleaved in a Ba fragment (60 kDa) and a Bb fragment (30 kDa). This antibody reacts with unactivated caiman complement factor B and the Bb fragment of activated factor B. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG2a/k |
| 免疫原： | Recombinant von Willebrandt domain of caiman factor B |
| 适用物种： | Ca |
| 应用范围： | E, WB |
| 应用说明： | Product Cat. No. 250444 reacts with caiman factor B in western blot, immunoprecipitation, and affinity purificatin. In ELISA however Product Cat. No. 250444 reacts with native caiman factor Bb or intact factor B. Caiman plasma can inhibit binding of the antibody to recombinant immunogen coated in microplate. E: 1:5,000; WB: 1:500 |
| 别名： | C3/C5 convertase; Properdin factor B; Glycine-rich beta glycoprotein; GBG; PBF2 |
| 形态： | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件： | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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文献和实验Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
. In this chapter, we describe 11-color high-dimensional (Hi-D) FACS staining and data analysis methods that provide greater clarity in identifying the B-cell subsets in bone marrow, spleen, and peritoneal cavity. Further, we show how a single Hi-D FACS antibody
by washing with 10 ml 50% Isopropanol, 25% Isopropanol, 10% Isopropanol, and 10 ml 1 mM ice cold HCl.(10) Resuspend the peptide or protein in 1 ml 1X Coupling Buffer and load the column. Hold at room temperature for 1 hour.(11) Wash with 5 ml 1X Coupling
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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