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- PEPROTECH
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- xy-189Hu01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Asialoglycoprotein Receptor 1 (ASGR1)
- 库存：
1000
- 供应商：
上海信裕
去唾液酸糖蛋白受体1(ASGPR1)重组蛋白
Recombinant Asialoglycoprotein Receptor 1 (ASGR1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy189Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 28.2kDa |
| Concentration | 5.8 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
去唾液酸糖蛋白受体1(ASGPR1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FSNF TASTEAQVKG LSTQGGNVGR KMKSLESQLE KQQKDLSEDH SSLLLHVKQF VSDLRSLSCQ MAALQGNGSE RTCCPVNWVE HERSCYWFSR SGKAWADADN YCRLEDAHLV VVTSWEEQKF VQHHIGPVNT WMGLHDQNGP WKWVDGTDYE TGFKNWRPEQ PDDWYGHGLG GGEDCAHFTD DGRWNDDVCQ RPYRWVCETE LDKASQEPP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FSNF TASTEAQVKG LSTQGGNVGR KMKSLESQLE KQQKDLSEDH SSLLLHVKQF VSDLRSLSCQ MAALQGNGSE RTCCPVNWVE HERSCYWFSR SGKAWADADN YCRLEDAHLV VVTSWEEQKF VQHHIGPVNT WMGLHDQNGP WKWVDGTDYE TGFKNWRPEQ PDDWYGHGLG GGEDCAHFTD DGRWNDDVCQ RPYRWVCETE LDKASQEPP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
去唾液酸糖蛋白受体1(ASGPR1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
去唾液酸糖蛋白受体1(ASGPR1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
去唾液酸糖蛋白受体1(ASGPR1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
受体介导的基因转移法 受体 介导的基因转移(receptor rmediated gene transfer)是利用细胞表面存在的特异性受体介导与配体结合的DNA内吞作用实现的。配体与受体的结合具有选择性，将DNA分子偶联在特异的配体上，借助配体与受体的选择性结合，可以实现靶基因的定向转移与表达。基于这种新的基因转移策略，目前已发展出了多种受体―配体系统，如脱唾液酸糖蛋白已被用于将目的基因靶向导人具有特异的脱唾液酸糖蛋白受体的肝细胞。脱唾液酸糖蛋白受体
，建议选择 OD 值落在标曲范围中部的稀释倍数为最佳稀释倍数。 2 回收率 评估试剂盒测量结果趋于真实值程度的指标。一般添加标准品（重组蛋白）到天然样本基质内，进行测定。通常，线性及回收率的范围在 80%-120% 比较合适。 3 天然样本检测 确保试剂盒可以检测天然样本及合适的样本类型。可根据靶蛋白性质对天然样本进行选择，例如：血清、血浆、细胞上清、尿液、唾液和乳汁等。 4 交叉反应 通过交叉反应评估试剂盒的特异性。交叉反应越小，说明试剂盒的特异性越好。 ✔与同家族其他蛋白或结构类似物的交叉
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去唾液酸糖蛋白受体1(ASGPR1)重组蛋白
¥1 - 3980
