- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-027Hu01
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)
- 库存：
1000
- 供应商：
上海信裕
泛素激活酶样蛋白(UBE1L)重组蛋白
Recombinant Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy027Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 10.3kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Lys429~Val488 linked by G with two N-terminal Tags, His-tag and T7-tag |
泛素激活酶样蛋白(UBE1L)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
KL RRQHYLLVGA GAIGCELLKV FALVGLGAGN SGGLTVVDMD HIERSNLSRQ FLFRSQDV G
KL RRQHYLLVGA GAIGCELLKV FALVGLGAGN SGGLTVVDMD HIERSNLSRQ FLFRSQDV G
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
泛素激活酶样蛋白(UBE1L)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
泛素激活酶样蛋白(UBE1L)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
泛素激活酶样蛋白(UBE1L)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
和 BCG-EST12 或 WT，NLRP3−/−，GSDMD−/−，caspase-1/11−/−BMDMs（I）感染 6 小时菌落计数。 结论： EST12 通过与巨噬细胞 RACK1 相互作用而引起细胞焦亡。RACK1 作为检测 M. tb EST12 的主要宿主因子，并招募去泛素酶 UCHL5 促进 NLRP3 去泛素化，进而激活巨噬细胞 NLRP3 炎性小体-caspase-1-GSDMD 细胞焦亡-IL-1β分子免疫通路。该研究阐释了巨噬细胞与 EST12 之间的相互作用，并证实了 EST
今天和小编一起关注更具独特性的抗体制备方法和抗体应用方向。 泛素和泛素化 泛素（ubiquitin，Ub）是一种小分子多肽，由76个氨基酸组成并广泛分布于真核细胞中。其主要功能是非底物特异性的标记需要降解的蛋白质，主要存在形式包括：游离的泛素、多泛素链、泛素化蛋白；不同形式存在的泛素含量反映了细胞的不同生理状态。 不同类型的泛素化 泛素化是指泛素分子在一系列特殊酶（如泛素激活酶、结合酶、连结酶、降解
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泛素激活酶样蛋白(UBE1L)重组蛋白
¥1 - 3980
