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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Matrix Metalloproteinase 8 (MMP8)
- 库存:
1000
- 供应商:
上海信裕
基质金属蛋白酶8(MMP8)重组蛋白
Recombinant Matrix Metalloproteinase 8 (MMP8)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy103Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 32.2kDa |
| Concentration | 6.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
基质金属蛋白酶8(MMP8)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR Q- LFLVAAHEF GHSLGLSHST DPGALMYPNY AYREPSTYSL PQDDINGIQT IYGPSDNPVQ PTGPSTPTAC DPHLRFDAAT TLRGEIYFFK DKYFWRRHPQ LRTVDLNFIS LFWPFLPNGL QAAYEDFDRD LVFLFKGRQY WALSAYDLQQ GYPRDISNYG FPRSVQAIDA AVSYNGKTYF FVNNQCWRYD NQRRSMDPGY PTSIASVFPG INCRIDAVFQ QDSFFLFFSG PQYFAFNLVS R
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEFELRR Q- LFLVAAHEF GHSLGLSHST DPGALMYPNY AYREPSTYSL PQDDINGIQT IYGPSDNPVQ PTGPSTPTAC DPHLRFDAAT TLRGEIYFFK DKYFWRRHPQ LRTVDLNFIS LFWPFLPNGL QAAYEDFDRD LVFLFKGRQY WALSAYDLQQ GYPRDISNYG FPRSVQAIDA AVSYNGKTYF FVNNQCWRYD NQRRSMDPGY PTSIASVFPG INCRIDAVFQ QDSFFLFFSG PQYFAFNLVS R
USAGE
基质金属蛋白酶8(MMP8)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
基质金属蛋白酶8(MMP8)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
基质金属蛋白酶8(MMP8)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
隆抗体蛋白产率的下限,即1-30μg/l08细胞/24小时。有人认为其限速步骤可嚣是在工程细胞中(对于重组蛋白来讲,常是异源的),重组蛋白的分泌效率较低。1、表达载体(1)表达栽体的类型哺乳动物细胞表达外源重组蛋白可利用质粒转染和病毒载体的感染。利用质粒转染获得稳定的转染细胞需几周甚至几个月时间,而利用病毒表达系统则可快速感染细胞,在几天内使外源基因整合到病毒载体中,尤其适用于从大量表达产物中检测出目的蛋白。根据进入宿主细胞的方式,可将表达载体分为病毒载体与质粒载体。病毒载体是以病毒颗粒的方式,通过病毒
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基质金属蛋白酶8(MMP8)重组蛋白
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